Effect of smoking on serum xanthine oxidase and malondialdehyde levels in patients with acute myocardial infarction

To evaluate the effect of smoking on blood levels of xanthine oxidase and malondialdehyde in acute myocardial infarction patients. The cross-sectional study was conducted from June to November 2013 and comprised myocardial infarction in-patients at Liaquat University of Medical and Health Sciences Hospital, Jamshoro, and Isra University Hospital, Hyderabad. Serum samples from age and gender matched smoking and non-smoking patients were investigated for the levels of xanthine oxidase and malondialdehyde by kit methods. Of the 88 serum samples investigated, 68[77.3%] belonged to men and 20[22.7%] to women. Among the 44 smokers, 35[79.5%] were men and 9[20.5%] were women. The overall mean age of the group was 49.6 +/- 8.0 years. Among the 44 non-smokers, 33[75%] were men and 11[25%] women. The overall mean age of the group was 50.9 +/- 8.4 Years. Smokers compared to non-smokers had significantly raised mean serum xanthine oxidase [0.31 +/- 0.05 vs 0.28 +/- 0.03mg/dl; p<0.05] and malondialdehyde [32.29 +/- 3.30 vs 30.30 +/- 2.87 micromol/L; p<0.04] levels. Smokers as against the non-smokers were at higher risk of developing acute myocardial infarction owing to increased level of oxidative stress caused by smoking

Evaluation of the effect of smoking on complete blood counts, serum C-reactive protein and magnesium levels in healthy adult male smokers

To examine the effect of smoking on complete blood count, serum C-reactive protein and magnesium levels in male smokers. The prospective case-control study was conducted in two villages of Matiari district in rural Sindh, Pakistan, from July to December 2013, and comprised healthy adult male smokers and an equal number of matching non-smokers as controls. The complete blood count, serum C-reactive protein and magnesium levels in all the subjects were measured to assess the effect of smoking on these parameters. The two groups had 48 subjects each with an overall age range of 20-40 years. The results of complete blood count were comparable except for lymphocyte, which was significantly higher [p<0.001], and neutrophil, which was lower [p<0.001] in smokers than in the non-smokers. Serum C-reactive protein concentrations among the cases [14.62 +/- 0.16mg/L] compared to the controls [4.81 +/- 0.38mg/L] were significantly higher [p<0.001]. However, reverse was true for serum magnesium levels which were significantly higher [p<0.001] in the controls [2.52 +/- 0.18mg/L] as against the cases [1.09 +/- 0.38mg/dl].Serum C-reactive protein-to-magnesium ratio was significantly higher [p<0.001] in smokers than in the non-smokers. Lymphocyte count was higher while neutrophil count was lower in smokers. Smoking also caused significant increase in serum C-reactive protein concentration concomitant to decrease in magnesium concentration in the smokers

Helicobacter pylori infection may be a new risk factor in developing acute myocardial infarction

The purpose of present study was to find out the relationship between helicobacter pylori infection and acute myocardial infarction Experimental / Case control study. This study was carried out the Biochemistry Department, Dow University of Health Sciences, Karachi from 11.02.2013 to 15.12.2013. Serum samples of age and gender matched 80 each of Cardiac and non-cardiac patients with H. Pylori were investigated for the levels of IgG and IgA by kit methods. Patients who were H.pyloric AMI compared to non-h.pyloric normal had relationship with increased IgG in Acute myocardial infarction patient with H.pyloric infection as compare to control. The results of present study suggest that H. pylori is a new risk factor for the development of atherosclerosis and enhance the risk factor. Therefore proper treatment and diagnosis can be helpful for cardiac patients